畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 2029-2035.doi: 10.11843/j.issn.0366-6964.2013.12.025

• 临床兽医 • 上一篇    

四君子汤对脾虚大鼠肠道菌群多样性的影响

孟良艳1,陈秀琴1,石达友1,黄海定1,2,郭世宁1*   

  1. (1.华南农业大学兽医学院,广州 510642;2.广东省中医院,广州 510405)
  • 收稿日期:2013-07-10 出版日期:2013-12-23 发布日期:2013-12-23
  • 通讯作者: 郭世宁,E-mail: shining@scau.edu.cn
  • 作者简介:孟良艳(1989-),女,内蒙古人,硕士生,主要从事临床兽医研究,E-mail:532128294@qq.com
  • 基金资助:

    十二五农村领域国家科技计划课程(2011BAD34B01-14);广东省教育部产学研合作项目(2011B09040026)

Influence of Sijunzi Decoction on Intestinal Flora Diversity in Spleen-deficient Rats

MENG Liang-yan1, CHEN Xiu-qin1, SHI Da-you1, HUANG Hai-ding1,2, GUO Shi-ning1*   

  1. (1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2.Laboratory of Animal Center, Guangzhou University of Traditional Medicine, Guangzhou 510405, China)
  • Received:2013-07-10 Online:2013-12-23 Published:2013-12-23

摘要:

探讨四君子汤对脾虚大鼠肠道菌群多样性的影响。将48只大鼠随机分为空白组、模型组、中药四君子汤组、柳氮磺吡啶肠溶片(SASP)组,每组12只。将除空白组外的其余各组大鼠用利血平法造脾虚模型。然后给四君子汤、SASP分别灌胃治疗后,通过16S rDNA测序法分析肠道菌群多样性变化。结果表明:与模型组比较,四君子汤组中的肠道菌群多样性明显升高,但与西药组中的肠道菌群多样性有差异。四君子汤能通过改善肠道菌群的多样性调理脾虚证。

Abstract:

The aim of this study was to investigate and analyze the recuperative function of Chinese Medicine, Sijunzi decoction, on intestinal flora in spleen-deficient animals. Forty-eight rats were randomly divided into the Control group (n=12), the Spleen-deficient group (n=12), the Sijunzi decoction group (n=12) and the Sulfasalazine group (n=12). Spleen-deficiency rat model was caused by reserpine in all groups other than the Control group. A diarrhea model of rats was conducted followed by treatment of sijunzi decoction or SASP or non-treatment. Intestinal flora was determined at pre-modeling, pre- and post- treatment by a 16S rDNA gene sequencing. The results showed that the Sijunzi decoction improved the diversity of intestinal flora in diarrhea rats though the diversity of intestinal flora differs from that treated with SASP. The Sijunzi decoction is a potential medicine for treatment of intestinal flora disorder induced diarrhea.

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